If this is a render out of MRQ then you need to set you PT sampling appropriately in there.
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this the only path tracing related sample settings. it’s in my post processing. I checked rest of my light setting, but they are ray tracing samples. not sure that they are same but still, they are set at 20 .
Path tracing samples set in post process are ignored by the movie render queue. You have to set it separately there. You need to enable anti aliasing in the movie render queue and set spatial samples to your desired sample count. There is also a info box in the path tracer section of the movie render queue that explains this a bit more.