Sprites Appear in Editor but Vanish when I Start the Game

Hello,
my sprites keep vanishing when I start the game even though they look fine in the viewport. This does not apply to the player character sprite, only to all the enemy sprites. Originally they didn’t appear at all, but now they appear for half a second, then vanish. I have no idea what I did to cause this, or why this is happening at all, everything was fine last night and all my sprites showed up without issue.

Does anyone know what might be causing this? Even a rough idea of what the source of the problem is would be a huge help

Video of what’s happening.