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Hello, I’ve was wondering if I could create multiple buttons in different style made with one png file?

I have a 500x500 .PNG file. Inside the PNG I have 4 buttons: button_small, button_small_pressed, button_big, button_big_pressed. Imgur: The magic of the Internet

I want to create now 2 buttons. Play (big) and exit (small).
Can I use 1 png for both buttons or do I have to create 4 png files?

thanks for reading