Thank you for posting in the community and I believe there isn't a limit but have asked for confirmation. I think because 425GB is way larger than the computer hardware can handle it will not work. I recommend working with 1GB-4GB files for best results with Twinmotion but larger files can work too. Since loading large information takes longer breaking down the 425GB in manageable data would be best.
Twinmotion uses Unreal Engine, thus you can reference the documentation to get more details on the hardware needs.