If you don’t have an official permission from the creators, whatever you do is done at your own risk; you may have problems or you may not. If your work is a completely non-commercial game (fan-art) it might be just fine; if it’s commercial you may or may not have problems (in some movies and TV series they have used others’ characters apparently without permission and with no problems, for example, but in some games there have been problems with funded mini-movies with characters and the ambientation of a released game franchise, as another example), so you should be very careful.
Maybe if your game is commercial the best solution (in legal safety terms) would be creating a character pretty similar to the one it’s inspiring it, but not identical, so people know that it’s a reference or parody but you don’t have to face legal problems.