Any way to toggle the "Use Splitscreen" option during gameplay?

if you need to toggle splitscreen in game, you will need to use c++.

GetWorld()->GetGameViewport()->SetDisableSplitscreenOverride(true);

if you want to expose it to blueprint, you can put it in a blueprint function library like this:

UABlueprintFunctionLibrary.h:

	UFUNCTION(BlueprintCallable, Category = "Viewport")
		static void DisableSplitScreen(AActor* Context, bool bDisable);

UABlueprintFunctionLibrary.CPP:

void UABlueprintFunctionLibrary::DisableSplitScreen(AActor* Context, bool bDisable)
{
	if (Context)
	{
		Context->GetWorld()->GetGameViewport()->SetDisableSplitscreenOverride(bDisable);
	}
}